UPC: 9781588292339 | Methods in Molecular Biology: Oligonucleotide Synthesis: Methods and Applications (Hardcover)

UPC lookup results for: 9781588292339 | Methods in Molecular Biology: Oligonucleotide Synthesis: Methods and Applications (Hardcover)

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity less costly DNA the synthesis of phosphorothioates using new sulfur transfer agents the synthesis of LNA peptide conjugation methods to improve cellular delivery and cell-specific targeting and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process nuclease footprinting to study the sequence-selective binding of small molecules of DNA nucleic acid libraries and the use of small interference RNA (siRNA) as an inhibitor of gene expression.